Coding

Part:BBa_K942009:Design

Designed by: Luis Mario Leal Garza   Group: iGEM12_Tec-Monterrey   (2012-09-26)


SS+ Zea m 14+His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 194


Design Notes

The SS at the N-terminal of this protein is useful for extracellular secretion. Its purification can be done by affinity to metallic ions with 6x His tag. Zea m 14 codon optimization is done to be better expressed in yeasts.


Source

In its native organism, Zea mays, Zea m 14 works as a lipid-transfer protein. It is known for causing allergenic response in humans.

References